Real-Time PCR course including qbase+ hands-on data analysis

8. - 9.11.2018 09:00 - 16:00 Brno, Czech Republic 500 € without VAT English Seats available: 0

Real-Time PCR – considered easy and straightforward if it comes to its hands-on part, has many drawbacks especially in experimental design and data analysis.
We focus in our course on all aspects of the Real-Time PCR workflow - planning your experiments, assay design and optimization, how to carry out basic as well as advanced data analysis and interpretation of results. Practical examples and demonstrations using software of Real-Time PCR instrument manufacturers as well as qbase+ (Biogazelle) will be used to demonstrate data analysis wokflow in gene expression studies including miRNA.

 

Who should attend?

The course is intended for everyone who plans to use Real-Time PCR or wishes to deepen their mastering of this technique. No preceding knowledge of Real-Time PCR is required. 

 

What will you learn?

  • How to design a successfull qPCR experiment including primers and probes
  • How to perform basic operations with qPCR data
  • How to efficiently handle software delivered together with qPCR instruments of various vendors
  • You will gain hands-on experience using various data sets and tools with a special focus on relative quantification and including endogeneous control selection
  • You will have the option of networking with an experienced speaker and other participants

 

And what will you get?

  • The course fee includes Premium 2 months license of qbase+, the most powerful, flexible and user-friendly Real-Time PCR data-analysis software compatible with data obtained using all Real-Time PCR instruments. Course agenda as well as qbase+ algorithms are fully MIQE compatible.

This course is supported by Biogazelle.

 

During the course…

  • The trainer will provide lectures with follow-up hands-on exercises in order to have the participants learning and assessing their knowledge
  • All course participants will perform all steps of qPCR data analysis tasks themselves!

 


 

Course agenda:

  • Principles and use of Real-Time PCR
  • Basics of data analysis
    • amplification plots, how to obtain Cq values
    • melting curves analysis
  • Assay design and validation – design in silico
    • probes and unspecific dyes
    • repeats, SNPs, secondary structures
    • specificity
  • Optimization
    • standard and melting curves
    • amplification efficiency and how do I know it?
    • specificity, sensitivity
  • Quantification using standard curve
    • planning, conducting, analysing
  • Relative quantification of not only gene expression
    • quantification models
    • amplification efficiency correction
    • selection of reference genes
    • alternative strategies of normalization
    • data distribution, choosing the proper statistical test
    • p- value - interpretation of results
  • Practical recommendations
    • lab setup
    • instruments and software
    • dispensable and non-dispensable controls

 


 

Course Logistics

  • Language: English
  • Registration fee includes: Workshop materials, Lunches/Coffee breaks
  • Participants must have their own laptops for qbase+ installation and for installation of a software you use or plan to use to handle your real-time pcr instrument. Basic steps of data analysis will be demonstrated using software of instrument manufacturers (Applied Biosystems, BioRad, Qiagen etc.), advanced relative quantification data analysis will be perfomed using qbase+.
  • How to reach the Workshop venue - the city of Brno (in the eastern part of the Czech Republic) can be easily accessed by car/bus/train from various directions. Please let us know should you need help with your travel arrangements.
  • Please kindly notice that lodging, travel and other incidental expenses are the responsibility of the attendee and that no labwork is to be performed during the course.

Real-Time PCR course including qbase+ hands-on data analysis

When: 8. - 9.11.2018
Time: 09:00 - 16:00
Where: Brno, Czech Republic
Address: JIC INBIT, Kamenice 34, Brno
Price: 500 € without VAT
Language: English
Seats available: 0
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