How tos and FAQs

What primers do you use for taxonomic analysis (based on 16S rDNA gene and other regions)?

Various hypervariable gene regions can be used for sequencing taxonomic analysis. Selection of primer combinations that we use for the amplification of these regions is continuously expanded. You can find the current available primer sets in our Sample submission sheet.

Please notice that the success of PCR amplification depends heavily on the integrity and purity of DNA you provide! Degraded or fragmented DNA produces weak amplicons with amplification artifacts. DNA with humic acids or other contaminants that interfere with amplification will also produce poor results. Removal of RNA during DNA purification is preferred. 

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