How tos and FAQs

How do I order Next-Generation Sequencing?

All orders are placed through our web interface. To order analyzes, you must...
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Sample submission guidelines

Please always follow steps described in our Sample submission guidelines.
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Guidelines for Ready-to-Run libraries (Illumina)

Here are our requirements for accepting libraries prepared by our clients.
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Additional sample submission guidelines - PacBio sequencing

Please read these recommendations before submitting samples for PacBio sequencing.
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Additional sample submission guidelines - Oxford Nanopore sequencing

Please read these recommendations before submitting samples for Oxford Nanopore sequencing.
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Sample shipment guidelines

It depends what samples you have and how they are processed, but it is generally advisable to send samples cooled...
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How do I measure DNA/RNA concentration?

Please measure DNA/RNA concentration with a fluorescence-based measurement such as Qubit or Picogreen assay. These measurements tend to be specific for DNA/RNA.
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What primers do you use for taxonomic analysis (based on 16S rDNA gene and other regions)?

Different hypervariable regions of genes can be used for taxonomic analysis. The table below lists primer sets we use to amplify these target regions and expected product sizes.
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Can I copy-paste into the NGS order form?

Definitely yes. Create (in Excel, for example) a table with three columns: Sample Name, Concentration, Volume. The sample names must be unique...
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Should I provide any specific data about my samples when submitting NGS order?

Yes. When submitting an order you will be asked to specify concentration and volume of each sample you send ...
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What sequencing capacity should I allocate?

This does depend on many factors, namely number of samples you submit, average fragment length...
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What data will I get after my sequencing experiment is finished?

You can receive data (results) in any format you wish. However, if you have no specific requirements, you will receive from us the following standard sequencing outputs.
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Can you guarantee what amount of NGS sequencing data I will get?

No. We can only estimate it. Even if we do our best to obtain perfect sequencing data for your samples...
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What happens with my NGS samples in SEQme lab?

The first step is a quality control (concentration measurement, gel visualization, bioanalyzer etc.).
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What if my NGS samples do not pass quality control in SEQme lab?

The key to a successful NGS experiment is starting with a sufficient quantity of high quality material.
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Will the data I finally get be analyzed?

This does depend on what you order. All samples always undergo a primary data analysis ...
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Where and how long do you store my NGS results?

All NGS orders are processed on our website. Results will be made accessible in your user profile, including but not limited to raw data, analysis results, supplementary data etc. and can be downloaded from within.
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Subscribing sequencing services

Click on link New Prepayment in your account and complete the form to subscribe to our services. The amount to be prepaid is given without VAT, the system will add VAT automatically according to applicable regulations. Once you have confirmed your order, an advance payment request will be issued automatically which you can use to ...
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Other questions?

Do not hesitate to contact us
We will be glad to help you.

Petr Vácha
+420 606 153 030
ngs@seqme.eu

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