Metagenome genotyping

Incredible promotion for unbiased metagenome analysis – from 30 Euro per sample - Order online!

Balanced sequencing strategy for metagenome genotyping

Next-Generation Sequencing is a very efficient tool when analyzing species in a given environment, commonly referred to as metagenome analysis. NGS has become the approach of choice due to its ability to identify all species in tested samples and is based on sequencing of pre-amplified hypervariable regions (such as 16S, 18S or ITS), traditionally used for species identification, and comparing thus obtained sequences with reference databases.

We apply a well-balanced sequencing strategy in order to achieve desired results. We recommend and perform:

List of primers we use to amplify hypervariable regions

You can choose from:

SO-040 Metagenomic library - 1 library of up to 48 pooled samples

(Includes Sample QC, Amplicon generation for up to 48 samples using a single primer set, double indexing, 1x library prep + QC)

SO-050 Metagenome analysis, up to 48 samples

(Includes Sample QC, Amplicon generation for up to 48 samples using a single primer set, double indexing, 1x library prep + QC, 1x MiSeq 250nt paired-end sequencing using V2 chemistry, 10-30 million reads, demultiplexing)

SO-060 Metagenome analysis, up to 96 samples

(Includes Sample QC, Amplicon generation for up to 96 samples using a single primer set, double indexing, 2x library prep + QC, pooling, 1x MiSeq 250nt paired-end sequencing using V2 chemistry, 10-30 million reads, demultiplexing)

SO-070 Metagenome data analysis, up to 48 samples

(Includes Data QC, Paired end read merging, Dereplication (Clustering), FASTQ to FASTA conversion, Taxonomy analysis + visualization, Data analysis report)

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