CleanSeq

CleanSeq

Sequencing of PCR products including their enzymatic cleanup.

This is the protocol for sequencing of PCR products, which also includes their enzymatic cleanup. You send us a template that is not ready for sequencing and must be purified first.

You supply:

  • The template is unpurified PCR product or other suitable template. In a separate tube you also supply sequencing primer(s). 
  • Only if you wish to use our universal primers, you supply the template only.

We perform:

  • Enzymatic cleanup of your PCR products
  • Mixing of the clean products and a sequencing primer in optimal ratio
  • Sequencing reaction
  • Capillary electrophoresis of sequencing reaction products
  • Manual data analysis

Sample requirements - CleanSeq

Time to results:

  • Typically up to 24 hours (working days) from the moment we receive your samples.

What you can expect:

  • Length of read up to 1100 bp, data accuracy typically > 99 %
  • Universal primers provided free of charge
  • Results online in .ab1, fasta, and .phd.1 format
  • Automatic email message as soon as your results are ready to download
  • Permanent internal quality control, repeated runs for free
  • Storage of your samples for one week, of your results for two years
  • Experienced technical support

 

Please notice that count of primers you can use is limited (1 primer for every 8 samples).

 

 

Data analysis:

The evaluation of the results obtained by the CleanSeq protocol is performed manually by our specialists! Each sample file is checked before sending and re-analyzed as needed to get the most out of it for you. We recommend to your attention the articles in our Magazine, where we continuously address various aspects of this method, including the evaluation of the results.

 

Comparison of our sequencing protocols - download HERE.

 

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