HairpinSeq

Special sequencing protocol for hairpins, GC-rich and other problematic templates. The price includes sequencing from both directions.

You supply:

Plasmid or PCR product and two primers (all in separate tubes). If you wish to use our universal primers, supply the template only.

We perform:

Bidirectional sequencing reaction using a special sequencing chemistry and both primers you supply, electrophoresis and data analysis.

Time to results:

Typically up to 24 hours

What you can expect:

  • Length of read up to 1100 bp from both directions (forward and reverse), data accuracy typically > 99 %
  • Universal primers provided free of charge
  • Results on line in .ab1, fasta, and .phd.1 format
  • Automatic email message as soon as your results are ready to download
  • Permanent internal quality control, repeated runs for free
  • Storage of your samples for one week, of your results for one year
  • Experienced technical support

Why choose HairpinSeq protocol?

Plasmid with GC-rich stretch, StandardSeq protocol:

Same plasmid, HairpinSeq protocol:

How to prepare samples
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