How tos and FAQs

ReadySeq – Preparation of samples

Sample is a sequencing reaction prepared by using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems), cleaned and finally dissolved in at least 15 μl of Hi-Di formamide (Applied Biosystems). Samples must be delivered in 96-well plates (Standard type, not Fast), compatible with Applied Biosystems sequencers.

We recommend that immediately after finishing your sample preparation you send them on ice by using a courier service (mail or our drop boxes are not recommended, ready sequencing reactions must be stored on ice and analyzed fast).

Samples must be delivered in 96-well plates. Do not use parafilm.

We recommend you contact us before sending your first batch of samples!

Download sample prep guide in pdf.

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