How tos and FAQs

How do I order Next-Generation Sequencing?

All orders are placed through our web interface. To order analyzes, you must... Read more

Sample submission guidelines

Please always follow steps described in our Sample submission guidelines. Read more

Guidelines for Ready-to-Run libraries (Illumina only)

Here are our requirements for accepting libraries prepared by our clients. Read more

Additional sample submission guidelines - PacBio sequencing

Please read these recommendations before submitting samples for PacBio sequencing. Read more

Additional sample submission guidelines - Oxford Nanopore sequencing

Please read these recommendations before submitting samples for Oxford Nanopore sequencing. Read more

Sample shipment guidelines

It depends what samples you have and how they are processed, but it is generally advisable to send samples cooled... Read more

How do I measure DNA/RNA concentration?

Please measure DNA/RNA concentration with a fluorescence-based measurement such as Qubit or Picogreen assay. These measurements tend to be specific for DNA/RNA. Read more

What primers do you use for taxonomic analysis (based on 16S rDNA gene and other regions)?

Various hypervariable gene regions can be used for sequencing taxonomic analysis. Selection of primer combinations that we use ... Read more

Can I copy-paste into the NGS order form?

Definitely yes. Create (in Excel, for example) a table with three columns: Sample Name, Concentration, Volume. The sample names must be unique... Read more

Should I provide any specific data about my samples when submitting NGS order?

Yes. When submitting an order you will be asked to specify concentration and volume of each sample you send ... Read more

What sequencing capacity should I allocate?

This does depend on many factors, namely number of samples you submit, average fragment length... Read more

What data will I receive after my sequencing experiment has been finished?

You can receive data (results) in any format you wish. However, if you have no specific requirements, you will receive from us the following standard sequencing outputs. Read more

Can you guarantee what amount of NGS sequencing data I will get?

It depends on the type and course of order processing. Read more

What happens with my NGS samples in SEQme lab?

The first step is a quality control (concentration measurement, gel visualization, bioanalyzer etc.). Read more

What if my NGS samples do not pass quality control in SEQme lab?

The key to a successful NGS experiment is starting with a sufficient quantity of high quality material. Read more

Will the data I finally get be analyzed?

This does depend on what you order. All samples always undergo a primary data analysis ... Read more

Where and how long do you store my NGS results?

All NGS orders are processed on our website. Results will be made accessible in your user profile, including but not limited to raw data, analysis results, supplementary data etc. and can be downloaded from within. Read more

Subscribing sequencing services

Click on link New Prepayment in your account and complete the form to subscribe to our services. The amount to be prepaid is given without VAT, the system will add VAT automatically according to applicable regulations. Once you have confirmed your order, an advance payment request will be issued automatically which you can use to ... Read more