RNASeq, sometimes also known as whole transcriptome sequencing, is the sequencing of a sample's mRNA content. Notably, it is a method by which a point-in-time snapshot of the transcriptome can be obtained. Easy it sounds, difficult it gets! This is why we organize this workshop every year and 2024 is no exception.
Sekvenování PCR produktů nebo plazmidů, přečišťování, speciální protokoly.
Více informací
Od návrhu projektu až po analýzu dat, vysokokapacitní sekvenování à la carte.
Více informací
Kurzy a školení, analýza výsledků. Plánujete experimenty na bázi Real-Time PCR?
Více informací
Péče o DNA sekvenátory a PCR cyklery, nabídka reagencií.
Více informacíSpolečnost SEQme byla založena v roce 2012 jako poskytovatel komplexních služeb v oblasti DNA sekvenování a Real-Time PCR.
Zabýváme se zejména prováděním laboratorních analýz, pořádáním kurzů a workshopů, zaměřených na tyto techniky, a servisem přístrojového vybavení.
Více o nás![Emergence of Rare Bovine–Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic](/upload/publications/moutelikova.jpg)
Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children. We investigated genotypes of RVA isolated from 225 stool specimens collected from Czech patients. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%), and, surprisingly, G8P[8] (9.3%). This is the first time that bovine–human DS-1-like G8P[8] strains were detected at a high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects the establishment of a new RVA strain in the population requires the continuous monitoring of rotavirus epidemiology.
Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanolbutanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium.
No common, unique genetic markers applicable to classification and phylogenetics for significant genera within the Propionibacteriaceae family have been suggested yet. Therefore, the aim of the study was to propose those genes in the genera Acidipropionibacterium , Cutibacterium , Propionibacterium and Pseudopropionibacterium . These genera were recently elicited from the genus Propionibacterium through whole genomic analyses...
In this article, we describe a new species from the C. mandelburgeri species complex that has evolved a parallel morphology and ecology to an unrelated species from the C. missioneira species complex (C. celidochilus). The new species is a pelagic predator that feeds predominantly on fishes and together with C. celidochilus is the only known pelagic species in the large riverine genus Crenicichla. The new species is endemic solely to a small tributary (the Urugua-í) of the Middle Paraná River. Mitochondrial DNA phylogeny finds the new species nested within the widespread C. mandelburgeri. Reduced genome-representation ddRAD analyses, however, demonstrate that this new species is of a hybrid…
Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
Barley is one of the most important crops in the world. In this study, the hypothesis was that there were no differences between two groups of barley varieties with different but defined malting qualities, which was tested using RNA sequencing during selected stages of malting. In total, 919 differentially transcribed genes between the two barley groups were identified and annotated and after optimization and validation, five molecular markers were developed for use in barley breeding.
Pro přepravu vzorků využijte naše schránky na vzorky, které pravidelně vybírá svozová služba. Transport vzorků touto cestou je pro vás zdarma!
Vzorky musí být do schránek vkládány v uzavřené obálce/obalu podobně jako kdybyste je zasílali poštou.
Kde jsou schránky na vzorky?
Kdybych dostal zaplaceno pokaždé, když odpovídáme na otázku “Jak mám měřit koncentraci DNA nebo RNA na přípravu NGS knihovny?”, tak bych nepsal tento příspěvek. Byl bych někde na jachtě okolo řeckých ostrovů. Ale jelikož na žádné jachtě nejsem, říkal…
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Mnozí z našich klientů vyžadují provedení pravidelné inspekční (preventivní) prohlídky. Ať již mezi ně patříte či nikoliv, přemýšleli jste někdy nad tím, jak poznáte, že byla provedena správně?
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