Sequencing of plasmids or PCR fragments, cleaning, special protocols.
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From project design to data analysis, high-throughput sequencing à la carte.
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Courses and trainings, analysis of results. Are you planning Real-Time PCR experiments?
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Care of Applied Biosystems® DNA sequencers and Real-Time PCR cyclers.
Tell me moreSEQme was established in 2012 to provide full solutions in the field of DNA sequencing and Real-Time PCR.
We conduct sample analyses, organize courses and workshops focused on these techniques and provide instrument services.
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Plant-soil feedback (PSF) represents an important process affecting natural plant communities. While many previous studies demonstrated the variation in the intensity of PSF between species, the mechanisms driving these differences are still largely unexplored. The aim of the study was to explore the importance of species traits and species phylogenetic relationships on the intensity of plant-soil feedback. The results supported our expectation that plant-soil feedback may be affected by species phylogenetic relationships. In addition, we have shown that also plant traits and the effect of the conditioning plant on soil chemistry may be useful factors allowing us to predict the intensity of…
The raccoon (Procyon lotor) carnivore native to North America is a fast spreading, invasive species in the Europe now. The data on the occurrence of Cryptosporidium spp. and microsporidia in raccoons is limited to North America’s territory and is totally lacking in the case of their introduction to Europe. Therefore, the objective of this study was to investigate the occurrence of microparasites in the introduced raccoons. The results suggest a possible role of raccoons in the contamination of the environment, including urban areas, with pathogens of zoonotic significance.
A putative operon encoding the biosynthetic pathway for the cytotoxic cyanobacterial lipopeptides puwainphycins was identified in Cylindrospermum alatosporum. Bioinformatics analysis enabled sequential prediction of puwainaphycin biosynthesis. Published results provide the first insight into the biosynthesis of frequently occurring b-amino fatty acid lipopeptides in cyanobacteria, which may facilitate analytical assessment and development of monitoring tools for cytotoxic cyanobacterial lipopeptides.
Mimicry complexes typically consist of multiple species that deter predators using similar anti-predatory signals. Here, we report a new multi-order mimicry complex that includes at least 140 different putative mimics from four arthropod orders including ants, wasps, bugs, tree hoppers and spiders. All members of this mimicry complex were similarly effective at deterring predators - even mildly defended mimics were rarely eaten by a community of invertebrate and vertebrate predators both in the wild and during staged trials.
The complete nucleotide sequences of RNA1 and RNA2 of the Holandsky cerveny strain of currant latent virus (CuLV) were determined using next-generation sequencing. A sequencing library was prepared using a KAPA DNA Library Preparation Kit and processed using a HiSeq2500 system (Illumina) by SEQme.eu (Dobris, Czech Republic). Phylogenetic analysis confirms that CuLV is a new distinct member of the genus Cheravirus.
The use of antibiotics in animal husbandry contributes to the world wide problem of increasing antibiotic resistance in animal and human pathogens. Intensive animal production is considered an important source of antibiotic resistance genes released to the environment, while the contribution of smaller farms remains to be evaluated. This study shows that antibiotic resistance genes may be a stable part of the intestinal metagenome of cattle even if antibiotics are not used for growth stimulation, and that smaller dairy farms may also contribute to environmental pollution with antibiotic resistance genes.
Swim bladder inflammation (SBI) is an important disease of common carp fingerlings in Central Europe, its etiology beingascribed to multicellular proliferative stages of the myxozoan parasite. Due to the presence of emerging numbers of proliferative myxozoan blood stages at different carp culture sites in recent years we analysed cases of SBI, for the first time, using molecular diagnostics, to identify the myxozoan parasites present in diseased swim bladders.
We are very satisfied with the job done on our 3130xl by Michael Lastuvka. This is service exactly as it should be, He is the right man in the right place.
Vaclav
Spadia Lab, Novy Jicin, Czech Republic
Clearly, he has a wealth of knowledge and information that could be tapped into. My overall impression is that the instrument is now in the care of a good company, and one that will be able to offer expert advice in relation to all aspects associated with the instrument. I am not aware of any other company who can offer such an inclusive “one stop shop” for every aspect associated with the ABI 310.
Declan
Galway-Mayo Institute of Technology, Galway, Ireland
... you did excellent job, the data are simply great. After your notice that direct sequencing of chromosomal DNA may not yield optimal results I was very surprised by the data I received. Perfect read length and a strong signal. Hats off and many thanks, this has helped me a lot.
Tomas
Institute of Soil Biology, Budweis, Czech Republic
I have learnt plenty of useful information and practical tips. Thanks for the perfect qPCR course.
Jana
Institute of Microbiology, Prague, Czech Republic
At the planning stage, we have always been presented with a detailed proposal and possible alternatives. Application Specialist Petr Vácha has a deep knowledge of NGS methodologies and is able to prepare a very efficient and cost-effective sequencing plan. During our debates, I was surprised by his recent knowledge, which is not common with company representatives. We have always found affordable but, more importantly, the correct way to carry out our methodically very diverse experiments.
Maria
Institute of Botany, Prague, Czech Republic
Use our sample collection boxes for sample shipment. These are regularly being serviced by our carrier service and the shipment is therefore free of charge!
Please make sure your samples are packed (in envelopes, for example) before placing them into the sample collection box.
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Are you wondering why you should send samples to us? Please read our 10 good reasons to answer this question!
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Have you ever seen the Applied Biosystems 7900HT Real-Time PCR system? Without really knowing what you are looking at, you probably didn’t like it. In my opinion this is the ugliest qPCR instrument ever, grey, huge, heavy and noisy.
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