Sequencing of plasmids or PCR fragments, cleaning, special protocols.
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From project design to data analysis, high-throughput sequencing à la carte.
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Courses and trainings, analysis of results. Are you planning Real-Time PCR experiments?
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Care of Applied Biosystems® DNA sequencers and Real-Time PCR cyclers.
Tell me moreSEQme was established in 2012 to provide full solutions in the field of DNA sequencing and Real-Time PCR.
We conduct sample analyses, organize courses and workshops focused on these techniques and provide instrument services.
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A putative operon encoding the biosynthetic pathway for the cytotoxic cyanobacterial lipopeptides puwainphycins was identified in Cylindrospermum alatosporum. Bioinformatics analysis enabled sequential prediction of puwainaphycin biosynthesis. Published results provide the first insight into the biosynthesis of frequently occurring b-amino fatty acid lipopeptides in cyanobacteria, which may facilitate analytical assessment and development of monitoring tools for cytotoxic cyanobacterial lipopeptides.
Swim bladder inflammation (SBI) is an important disease of common carp fingerlings in Central Europe, its etiology beingascribed to multicellular proliferative stages of the myxozoan parasite. Due to the presence of emerging numbers of proliferative myxozoan blood stages at different carp culture sites in recent years we analysed cases of SBI, for the first time, using molecular diagnostics, to identify the myxozoan parasites present in diseased swim bladders.
The use of antibiotics in animal husbandry contributes to the world wide problem of increasing antibiotic resistance in animal and human pathogens. Intensive animal production is considered an important source of antibiotic resistance genes released to the environment, while the contribution of smaller farms remains to be evaluated. This study shows that antibiotic resistance genes may be a stable part of the intestinal metagenome of cattle even if antibiotics are not used for growth stimulation, and that smaller dairy farms may also contribute to environmental pollution with antibiotic resistance genes.
The raccoon (Procyon lotor) carnivore native to North America is a fast spreading, invasive species in the Europe now. The data on the occurrence of Cryptosporidium spp. and microsporidia in raccoons is limited to North America’s territory and is totally lacking in the case of their introduction to Europe. Therefore, the objective of this study was to investigate the occurrence of microparasites in the introduced raccoons. The results suggest a possible role of raccoons in the contamination of the environment, including urban areas, with pathogens of zoonotic significance.
Mimicry complexes typically consist of multiple species that deter predators using similar anti-predatory signals. Here, we report a new multi-order mimicry complex that includes at least 140 different putative mimics from four arthropod orders including ants, wasps, bugs, tree hoppers and spiders. All members of this mimicry complex were similarly effective at deterring predators - even mildly defended mimics were rarely eaten by a community of invertebrate and vertebrate predators both in the wild and during staged trials.
The complete nucleotide sequences of RNA1 and RNA2 of the Holandsky cerveny strain of currant latent virus (CuLV) were determined using next-generation sequencing. A sequencing library was prepared using a KAPA DNA Library Preparation Kit and processed using a HiSeq2500 system (Illumina) by SEQme.eu (Dobris, Czech Republic). Phylogenetic analysis confirms that CuLV is a new distinct member of the genus Cheravirus.
Plant-soil feedback (PSF) represents an important process affecting natural plant communities. While many previous studies demonstrated the variation in the intensity of PSF between species, the mechanisms driving these differences are still largely unexplored. The aim of the study was to explore the importance of species traits and species phylogenetic relationships on the intensity of plant-soil feedback. The results supported our expectation that plant-soil feedback may be affected by species phylogenetic relationships. In addition, we have shown that also plant traits and the effect of the conditioning plant on soil chemistry may be useful factors allowing us to predict the intensity of…
Interactive collaboration has been very good overall, starting from the nucleic acid quality control, through the preparation of libraries and to sequencing results. The main advantage of SEQme is that you can directly discuss various individual issues and eliminate them, which is hardly possible in other sequencing companies.
Josef
Biology Centre, Budweis, Czech Republic
I have learnt plenty of useful information and practical tips. Thanks for the perfect qPCR course.
Jana
Institute of Microbiology, Prague, Czech Republic
Clearly, he has a wealth of knowledge and information that could be tapped into. My overall impression is that the instrument is now in the care of a good company, and one that will be able to offer expert advice in relation to all aspects associated with the instrument. I am not aware of any other company who can offer such an inclusive “one stop shop” for every aspect associated with the ABI 310.
Declan
Galway-Mayo Institute of Technology, Galway, Ireland
... you did excellent job, the data are simply great. After your notice that direct sequencing of chromosomal DNA may not yield optimal results I was very surprised by the data I received. Perfect read length and a strong signal. Hats off and many thanks, this has helped me a lot.
Tomas
Institute of Soil Biology, Budweis, Czech Republic
I am very thankful for the great Real-Time PCR course!
Tomas
Institute of Experimental Oncology, Bratislava, Slovakia
Use our sample collection boxes for sample shipment. These are regularly being serviced by our carrier service and the shipment is therefore free of charge!
Please make sure your samples are packed (in envelopes, for example) before placing them into the sample collection box.
Show box locations
In a relative quantification experiment, what you are most likely interested in is to compare the expression level of a particular gene among different samples. The most common way of correcting variations in the target nucleic acids input amount among…
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We recently had the opportunity to buy MiSeq, not from Illumina, but from the previous owner. At first, it looked like an interesting offer, but it became a little nightmare. So, I thought I would try to share experiences. Maybe somebody knows somebody…
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