© SEQme s.r.o., 2012 - 2021. All rights reserved.
Disclaimer.
webdesign Beneš & Michl
Our courses can be organized in your lab and their agenda tailored exactly to your needs. And in case of pending covid-restrictions may go online or be cancelled as well...
This workshop is aimed at scientists who want to learn more about working with data obtained using Oxford Nanopore with a special focus on hybrid de novo assembly using Illumina + Oxford Nanopore reads. During the workshop, you will independently perform your own de novo assembly using the provided data files.
The aim of the course is to help you gain a basic orientation in technologies, principles, concepts and possibilities of using Next-Generation sequencing (NGS). Emphasis is placed on mastering the ability of independent effective planning of NGS experiments and basic evaluation of results. We will discuss the whole topic comprehensively from A to Z in context.
You will get acquainted with various formats of NGS data, you will learn about specific advantages and disadvantages of used sequencing technologies and how to deal with them, and you will understand the background of individual processes used to modify NGS data. After this three-day workshop, you will not become a bioinformatician, but you will be well on your way to becoming a qualified partner for bioinformaticians in your team.
Detection and identification - so-called variant calling in sequencing data is in principle a simple matter, which in practice is far from simple and the distinction between real variant (mutation) and experimental error requires sophisticated computational and / or heuristic techniques. In our workshop you will learn exactly these procedures.
Assembly of the transcriptome based on RNAseq data is a powerful tool for detecting changes in gene expression. However, the ability to perform such analyzes accurately depends crucially on the quality of the assembly. Our workshop is focused on bioinformatics processing of RNA-Seq data and transcriptome assembly. We will guide you through data quality control, filtering, efficient and accurate correction of sequencing errors, assembly of the transcriptome and its evaluation.
If you are performing an RNA-Seq experiment, it is important that you understand how to access your dataset, how to consider its specifics, and how to track its weaknesses that may limit your ability to draw the right conclusions. The aim of our workshop is for you to master the bioinformatics procedures of RNA-Seq data processing.
Metagenomics is the study of genetic material obtained directly from environmental samples. This is useful when trying to understand what microbes are present in a sample and what they do in a particular environment. In our workshop you will learn how to process metagenomic data ranging from quality control, filtering, assembly / clustering to taxonomic classification, functional classification and comparative metagenomics.
Recently, Next-Generation sequencing technologies have been used extensively for virus detection purposes. They are absolutely essential for agricultural production and human health. Probably the most advanced method of this detection is based on the use of shotgun sequencing, subsequent assembly and virus identification. Our workshop is focused on this analytical procedure.
Real-Time PCR training to make you trust your machine, your data and your operating procedures. The fastest way to use this technique effectively. Any Real-Time PCR instrument - Applied Biosystems, Bio-Rad, Corbett Research, Roche, Stratagene/Agilent, Eppendorf, Illumina, Qiagen etc. Duration: 1-2 days or as neededIn Czech or English, in your lab
Training for current or new instrument / software users. In your lab, using your instrument and software versions. You will get exactly the information you need. Sequencers / genetic analyzers Applied Biosystems 310, 3100, 3130/xl, 3500/xl, 3730/xl. In Czech or English, 1-3 days or as need be. On a one-time basis or regularly in labs maintaining highest standards of training of personnel. Expert training for expert users.