Introduction to Next-Generation Sequencing Course

20. - 21.5.2019 08:30 - 17:00 Brno, Czech Republic 350 € without VAT English Seats available: 23

Get deeper insight into the world of massive parallel sequencing and learn how to plan and conduct your own experiments effectively!
The course is designed for molecular biologists interested in Next-Generation Sequencing (NGS) technology. Prerequisite is computer literacy and basic knowledge of molecular biology (DNA, RNA, gene expression, PCR).

The aim of the course is to help you acquire basic orientation in technologies, principles, concepts and possibilities of using NGS. Emphasis is placed on acquiring the ability of independent EFFECTIVE planning of NGS experiments and basic evaluation of results.

Please note: If you want to learn the basics of NGS data analysis, we would like to draw your attention to our next event - Next-Generation Sequencing data analysis – Workshop for beginners!, which is a follow-up to this two-day course.

 

Who should attend?

The course is intended for everyone who plans to use technologies of massively-parallel (next-generation) sequencing or wishes to deepen their mastering of these techniques. No preceding knowledge of sequencing technologies is required. 

 

What will you learn?

  • How to design a successfull NGS experiment including its budget planning
  • How to perform basic operations with NGS data including information about the computer infrastructure
  • You will gain an overview of various NGS applications
  • You will have the option of networking with experienced speakers and other participants

 


 

Course agenda:

  • Next-Generation sequencing - Introduction
    • Overview, history and development of sequencing techniques
    • Basic terms and principles
    • NGS applications and workflow
  • NGS technologies and platforms
    • Technological overview
    • Illumina, Thermo Fisher (IonTorrent) and Pacific Biosciences – how they work
    • And what’s really new?
  • Standard applications and techniques
    • Overview
    • Library preparation
    • Target enrichment
  • Experiment planning
    • Your goals and experimental design
    • Application-specific requirements
    • Technical design
  • NGS data analysis
    • Overview, data analysis environments and bioinformatic workflows
    • NGS data files and formats
    • Quality assessment and data pre-processing
    • Software overview
    • Secondary data analysis (de-novo genome assembly, resequencing, variant calling, …)
    • Evaluation of results and statistics
    • Visualization of results

 


 

Course Logistics:

  • Language: English
  • Registration fee includes: Workshop materials, Lunches/Coffee breaks
  • How to reach the Workshop venue - the city of Brno (in the eastern part of the Czech Republic) can be easily accessed by car/bus/train from various directions. Please let us know should you need help with your travel arrangements.
  • Please kindly notice that lodging, travel and other incidental expenses are the responsibility of the attendee and that no labwork is to be performed during the course.

 


Introduction to Next-Generation Sequencing Course

When: 20. - 21.5.2019
Time: 08:30 - 17:00
Where: Brno, Czech Republic
Address: JIC INBIT, Kamenice 34, Brno
Price: 350 € without VAT
Language: English
Seats available: 23
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