Real-Time PCR

Magazine


Is there a need for the reference gene(s) to be measured in the same plate (run) as the gene of interest?

In a relative quantification experiment, what you are most likely interested in is to compare the expression level of a particular gene among different samples. The most common way of correcting variations in the target nucleic acids input amount among samples is ...



Do you own Applied Biosystems 7900HT? Lucky you!

Have you ever seen the Applied Biosystems 7900HT Real-Time PCR system? Without really knowing what you are looking at, you probably didn’t like it. In my opinion this is the ugliest qPCR instrument ever, grey, huge, heavy and noisy.


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