Next-Gen sequencing

Magazine


Trying to buy MiSeq second hand ...

We recently had the opportunity to buy MiSeq, not from Illumina, but from the previous owner. At first, it looked like an interesting offer, but it became a little nightmare. So, I thought I would try to share experiences. Maybe somebody knows somebody who can find someone who can ...?



New horizons for Next-Generation Sequencing with GemCode technology (10x Genomics)

Our constant search for the best and most appropriate solutions to various experimental designs has led us to the introduction of the GemCode technology into our portfolio of Next-Generation sequencing services. In this post, we are happy to announce its availability to our clients and provide its basic description.



Several good reasons why Qubit is better than Nanodrop when quantifying your samples for NGS library prep

If I had a nickel for every time we answered the “How should I quantify my DNA or RNA for NGS library prep?” question, I wouldn’t need to be writing this article. I’d be on a boat in the Mediterranean. But since I’m not on a boat anywhere, I thought I could perhaps collect several good reasons why using Nanodrop is not really the best way of doing this.



A few thoughts on data analysis

The technology of next-generation sequencing produces huge amounts of data compared to Sanger technology. Its volume naturally depends on the design of the experiment, but primarily on the output capacity of the instrument. In principle, it is always necessary to deal with the transfer of large amounts of data into a form which enables their effective processing to allow a deeper analysis of the sequences obtained which is the very aim of the experiment.



Short Glossary of NGS terms

Brief explanation of commonly used NGS terms


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