StandardSeq
CleanSeq
HairpinSeq
ReadySeq
Fragment Analysis
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Please use ACGTN symbols for the primer sequences, IUPAC codes (BDHRYKMSWV) for degenerate bases and “I” for Inosine.
If there are any incomplementary overlaps at 5’ ends of primers (e.g. with a restriction site), please enter only the portion of the primer sequence which is complementary to the sequenced template.