How tos and FAQs

CleanSeq – Preparation of samples

Sample is an unpurified PCR product (aliquot of the PCR reaction) or other suitable template, amenable to enzymatic cleanup. You must also supply sequencing primer(s) or select some of our universal primers.

 

Primer requirements:

  • Primer volume: 200 μl per 100 samples.
  • Primer concentration: 5 μM.
  • Please note that up to six sequencing primers can be used if samples are supplied in the plate. If samples are supplied in tubes, one primer can be used for each 16 samples ordered.

 

Sample requirements:

Template

Concentration

Total volume

PCR product <500 bp

~10 ng/μl

5-10 μl

PCR product 500–1000 bp

~20 ng/μl

PCR product >1000 bp

~40 ng/μl

Plasmid

~100 ng/μl

 

Note: If you want to order bidirectional sequencing, it is just enough to send for example a single plate with 96 samples, each having a volume of 5-10 μl, and two sequencing primers. But it is necessary to order sequencing of two plates - the first plate by the CleanSeq protocol (using one of primers) and the other by the StandardSeq protocol (using the other primer).

Please do not use parafilm or adhesive tape to secure your plasticware.

Download sample prep guide in pdf.

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