How tos and FAQs

ReadySeq – Preparation of samples

Sample is a sequencing reaction prepared by using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems), cleaned and finally dissolved in at least 15 μl of Hi-Di formamide (Applied Biosystems). Samples must be delivered in 96-well plates (Standard type, not Fast), compatible with Applied Biosystems sequencers.

We recommend that immediately after finishing your sample preparation you send them on ice by using a courier service (mail or our drop boxes are not recommended, ready sequencing reactions must be stored on ice and analyzed fast).

Please do not use parafilm or adhesive tape to secure your plasticware.

We recommend you contact us before sending your first batch of samples!

Download sample prep guide in pdf.

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